In-situ identification of major metabolites in the red alga Gracilariopsis lemaneiformis using high-resolution magic angle spinning nuclear magnetic resonance spectroscopy

Abstract

The content of low-molecular-weight compounds in the red alga Gracilariopsis lemaneiformis [(Bory) Dawson, Acleto, et Foldvik] has been analysed in-situ using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. The major heteroside was shown to be floridoside, but digeneaside and isofloridoside were also detected in the alga. Other major components were isethionic acid and the amino acids taurine and citrulline. The results from the HR-MAS NMR analysis were confirmed with high-resolution NMR spectroscopy, high-resolution fast atom bombardment mass spectrometry (FABMS) and GC-MS, on material isolated from the studied alga, but also on authentic samples.

Keywords

digeneaside; floridoside; Gracilariopsis; high-resolution magic angle spinning; isethionic acid; isofloridoside

Published in

Planta
1998, volume: 206, number: 2, pages: 300-307

SLU Authors

• Broberg, Anders

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Kenne, Lennart

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Organic Chemistry
Analytical Chemistry

Publication identifier

• DOI: https://doi.org/10.1007/s004250050404

Permanent link to this page (URI)

https://res.slu.se/id/publ/87621