Fluctuations in ribosomal RNA gene content and nucleolar activity in the cambial region of Abies balsamea (Pinaceae) shoots during reactivation

Abstract

Tissue was collected from the vascular cambial region of 1-year-old balsam fir shoots over an Ii-week period during which cambial reactivation occurred. The amount of rDNA (ribosomal RNA genes) relative to total genomic DNA was determined by quantitative slot blots for three trees, one of which showed a 3-week delay in reactivation. In addition, nucleolar activity was estimated by measuring nucleolar volume, number, and staining intensity. Relative rRNA gene content increased transiently prior to the onset of cambial cell periclinal division. Nucleolar volume also increased transiently, but 1-2 weeks prior to the maximal relative rDNA value. The increases in relative rDNA and nucleolar activity were delayed in the tree in which reactivation was late. We interpret these changes as reflecting the amplification and loss of genes encoding rRNA to facilitate cambial cell reactivation.

Published in

American Journal of Botany
1994, volume: 81, number: 11, pages: 1384-1389
Publisher: BOTANICAL SOC AMER INC

SLU Authors

• Mellerowicz, Ewa

  • University of New Brunswick

UKÄ Subject classification

Forest Science

Publication identifier

• DOI: https://doi.org/10.2307/2445309

Permanent link to this page (URI)

https://res.slu.se/id/publ/88949