Sampels, Sabine
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- University of South Bohemia in České Budějovice
Abstract
The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0-5 mu M) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR.The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected. The presence of the chosen fatty acids did not affect the uptake of either cadmium or iron, zinc and copper. The presence of already 1 mu M cadmium increased the mRNA expression of mt1 g and mt1 m significantly, while the fatty acids did not interfere with the effect of cadmium.
Keywords
EPA; DHA; Hepatoblastoma cells; mt1 g; mt1 m; Cadmium
Published in
Toxicology in Vitro
2017, volume: 44, pages: 372-376
SLU Authors
UKÄ Subject classification
Food Science
Nutrition and Dietetics
Publication identifier
- DOI: https://doi.org/10.1016/j.tiv.2017.08.003
Permanent link to this page (URI)
https://res.slu.se/id/publ/89265