Transglutaminase from newly isolated Streptomyces sp. CBMAI 1617: production optimization, characterization and evaluation in wheat protein and dough systems.

Ceresino, Elaine B.; de Melo, Ricardo R.; Kuktaite, Ramune; Hedenqvist, Mikael S.; Zucchi, Tiago D.; Johansson, Eva; Sato, Helia H.

doi:10.1016/j.foodchem.2017.09.010

Abstract

The popularity of transglutaminase (TG) by the food industry and the variation in functionality of this enzyme from different origins, prompted us to isolate and evaluate a high-yielding TG strain. Through the statistical approaches, Plackett-Burman and response surface methodology, a low cost fermentation media was obtained to produce 6.074 +/- 0.019 U mL(-1) of TG from a novel source; Streptomyces sp. CBMAI 1617 (SB6). Its potential exploitation was compared to commonly used TG, from Streptomyces mobaraensis. Biochemical and FT-IR studies indicated differences between SB6 and commercial TG (Biobond (TM) TG-M). Additions of TG to wheat protein and flour based doughs revealed that the dough stretching depended on the wheat protein fraction, TG amount and its origin. A higher degree of cross-linking of glutenins and of inclusion of gliadin in the polymers was seen for SB6 as compared to commercial TG. Thus, our results support the potential of SB6 to tailor wheat protein properties within various food applications.

Keywords

Transglutaminase; Enzyme production; Wheat protein polymers; Cross-linking

Published in

Food Chemistry
2018, volume: 241, pages: 403-410

SLU Authors

Ceresino, Elaine
- University of Campinas
Kuktaite, Ramune
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Johansson, Eva
- Department of Plant Breeding, Swedish University of Agricultural Sciences

UKÄ Subject classification

Food Science
Agricultural Science

Publication identifier

DOI: https://doi.org/10.1016/j.foodchem.2017.09.010

Permanent link to this page (URI)

https://res.slu.se/id/publ/90301