Development of a rapid and simple Agrobacterium tumefaciens-mediated transformation system for the fungal pathogen Heterobasidion annosum

Abstract

Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 degrees C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.

Keywords

Agrobacterium tumefaciens; Heterobasidion annosum; green fluorescent protein (GFP); transformation

Published in

FEMS Microbiology Letters
2006, volume: 255, number: 1, pages: 82-88
Publisher: BLACKWELL PUBLISHING

SLU Authors

• Samils, Nicklas

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Elfstrand, Malin

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Fahleson, Jan

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Olson, Åke

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Dixelius, Christina

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Stenlid, Jan

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Forest Science

Publication identifier

• DOI: https://doi.org/10.1111/j.1574-6968.2005.00069.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/9081