A standardized toolkit for genetic engineering of CTG Glade yeasts

Abstract

We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG Glade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.

Keywords

Candida; Synthetic construct; Standardization; beta-galactosidase; Fluorescent proteins; Luciferase

Published in

Journal of Microbiological Methods
2018, volume: 144, pages: 152-156

SLU Authors

• Linder, Tomas

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics and Genomics
Microbiology

Publication identifier

• DOI: https://doi.org/10.1016/j.mimet.2017.11.015

Permanent link to this page (URI)

https://res.slu.se/id/publ/92152