Simura, Jan
- The Czech Academy of Sciences
Abstract
Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.
Published in
Plant Physiology
2018, volume: 177, number: 2, pages: 476-489
Publisher: AMER SOC PLANT BIOLOGISTS
SLU Authors
Antoniadi, Ioanna
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Ljung, Karin
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Novak, Ondrej
- The Czech Academy of Sciences
UKÄ Subject classification
Botany
Publication identifier
- DOI: https://doi.org/10.1104/pp.18.00293
Permanent link to this page (URI)
https://res.slu.se/id/publ/95887