Khan Kayani, Waqas
- University of Poonch Rawalakot
Abstract
Lettuce is an important edible crop which possesses various medicinal properties. In this study Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene. Transgene integration and expression was confirmed through PCR and semiquantitative RT-PCR. The transformed extracts were evaluated for their in vitro antioxidant and in vivo analgesic, anti-inflammatory and antidepressant activities in rats. The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants. Results of total reducing power and total antioxidant capacity exhibited 90-118 and 61-75 % increase in transformed plants, respectively. In contrast to control, DPPH, lipid peroxidation and DNA protection assay showed up to 37, 20 and 50 % enhancement in transformed plants, respectively. The extracts showed similar but significant enhancement behavior in hot plate analgesic and carrageenan-induced hind paw edema test. The transformed extracts showed 72.1 and 78.5 % increase for analgesic and anti-inflammatory activities, respectively. The transformants of rol C gene exhibited prominent antidepressant activity with 64-73 % increase compared with untransformed plants. In conclusion, the present work suggests that transformation with rol C gene can be used to generate lettuce with enhanced medicinally important properties, such as antioxidant, analgesic, anti-inflammatory and antidepressant potential.
Keywords
Agrobacterium tumefaciens; Analgesic; Antiinflammatory; Antidepressant; Antioxidants; Lactuca sativa; rol genes; Semiquantitative PCR
Published in
3 BIOTECH
2016, volume: 6, article number: 215
SLU Authors
UKÄ Subject classification
Plant Biotechnology
Publication identifier
- DOI: https://doi.org/10.1007/s13205-016-0533-4
Permanent link to this page (URI)
https://res.slu.se/id/publ/98961