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Abstract

There is an increasing importance for using biocontrol agents in combating plant diseases sustainably and in the long term. As large scale genomic sequencing becomes economically viable, the impact of single nucleotide polymorphisms (SNPs) on biocontrol-associated phenotypes can be easily studied across entire genomes of fungal populations. Here, we improved a previously reported genome assembly of the biocontrol fungus Clonostachys rosea strain IK726 using the PacBio sequencing platform, which resulted in a total genome size of 70.7 Mbp and 21,246 predicted genes. We further performed whole-genome re-sequencing of 52 additional C. rosea strains isolated globally using Illumina sequencing technology, in order to perform genome-wide association studies in conditions relevant for biocontrol activity. One such condition is the ability to grow at lower temperatures commonly encountered in cryic or frigid soils in temperate regions, as these will be prevalent for protecting growing crops in temperate climates. Growth rates at 10 degrees C on potato dextrose agar of the 53 sequenced strains of C. rosea were measured and ranged between 0.066 and 0.413 mm/day. Performing a genome wide association study, a total of 1,478 SNP markers were significantly associated with the trait and located in 227 scaffolds, within or close to (

Keywords

biocontrol; Clonostachys rosea; comparative genomics; genome sequencing; GWAS

Published in

Frontiers in Microbiology
2018, volume: 9, article number: 2844

SLU Authors

Associated SLU-program

SLU Plant Protection Network

UKÄ Subject classification

Agricultural Science
Genetics and Genomics

Publication identifier

  • DOI: https://doi.org/10.3389/fmicb.2018.02844

Permanent link to this page (URI)

https://res.slu.se/id/publ/99872