Abstract

We investigated the metabolic and cellular stress responses in an endemic catfish Horabagrus brachysoma acclimated to ambient (26 degrees C), 31, 33 and 36 degrees C for 30 days. After acclimation, fish were sampled to investigate changes in the levels of blood glucose, tissue glycogen and ascorbic acid, activities of enzymes involved in glycolysis (LDH), citric acid cycle (MDH), gluconeogenesis (FBPase and G6Pase), pentose phosphate pathway (G6PDH), protein metabolism (AST and ALT), phosphate metabolism (ACP and ALP) and energy metabolism (ATPase), and HSP70 levels in various tissues. Acclimation to higher temperatures (33 and 36 degrees C) significantly increased activities of LDH, MDH, ALP, ACP, AST, ALT and ATPase and blood glucose levels, whereas decreased the G6PDH enzyme activity and, tissue glycogen and ascorbic acid. Results indicated an overall increase in the carbohydrate, protein and lipid metabolism implying increased metabolic demands for maintaining homeostasis in fish acclimated to higher temperatures (33 and 36 degrees C). We observed tissue specific response of HSP70 in H. brachysoma, with significant increase in gill and liver at 33 and 36 degrees C, and in brain and muscle at 36 degrees C, enabling cellular protection at higher acclimation temperatures. In conclusion, H. brachysoma adjusted metabolic and cellular responses to withstand increased temperatures, however, these responses suggest that the fish was under stress at 33 degrees C or higher temperature.

Keywords

Thermal acclimation; Horabagrus brachysoma; Metabolic enzymes; Secondary stress responses; HSP70

Published in

Journal of Thermal Biology
2017, Volume: 65, pages: 32-40
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

UKÄ Subject classification

Fish and Aquacultural Science


Publication identifier

DOI: https://doi.org/10.1016/j.jtherbio.2017.02.003

Permanent link to this page (URI)

https://res.slu.se/id/publ/100066