Abstract

Like other invertebrates, the brine shrimp Artemia franciscana relies solely on innate immunity, which by definition lacks adaptive characteristics, to combat against invading pathogens. One of the innate mechanisms is melanisation of bacteria mediated by the activation of the prophenoloxidase (proPO) system. The 70 kDa heat shock proteins (Hsp70) derived from either prokaryote (Escherichia coli) or eukaryote (Artemia), well conserved and immune-dominant molecules, protect Artemia against Vibrio campbellii. However, the molecular mechanisms by which these proteins protect Artemia against Vibrio campbellii infection are unknown. Here we demonstrated that feeding gnotobiotically grown Artemia with either Artemia Hsp70 or the E. coli Hsp70 equivalent DnaK, each overproduced in E. coli, followed by V campbellii challenge enhanced the proPO system, at both mRNA and protein activity levels. Additionally, the Artemia fed with these proteins survived well in a Vibrio challenge assay. These results indicated that Hsp70s derived from either prokaryotic or eukaryotic sources generate protective immunity in the crustacean Anemia against V. campbellii infection by priming the proPO system. This is apparently the first in vivo report on priming activity of Hsp70 in an invertebrate. (C) 2011 Elsevier Ltd. All rights reserved.

Keywords

Heat shock protein (Hsp) 70; Phenoloxidase gene; Priming; Artemia franciscana; Vibrio campbellii

Published in

Fish and Shellfish Immunology
2011, Volume: 31, number: 1, pages: 134-141
Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

UKÄ Subject classification

Fish and Aquacultural Science


Publication Identifiers

DOI: https://doi.org/10.1016/j.fsi.2011.04.008

Permanent link to this page (URI)

https://res.slu.se/id/publ/100075