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Research article2019Peer reviewedOpen access

A high-throughput amplicon-based method for estimating outcrossing rates

Jantzen, Friederike; Wozniak, Natalia; Kappel, Christian; Sicard, Adrien; Lenhard, Michael


BackgroundThe outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCR-genotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.ResultsWe have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd's Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons.ConclusionsThis novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.


Outcrossing; Mixed mating; Outcrossing rate; Capsella; Amplicon sequencing

Published in

Plant Methods
2019, Volume: 15, article number: 47
Publisher: BMC

    UKÄ Subject classification

    Biochemistry and Molecular Biology

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