Candidate pheromone receptors of codling moth Cydia pomonella respond to pheromones and kairomones

Abstract

Olfaction plays a dominant role in the mate-finding and host selection behaviors of the codling moth (Cydia pomonella), an important pest of apple, pear and walnut orchards. Antennal transcriptome analysis (Bengtsson et al. 2012, Walker et al. 2016) revealed a number of abundantly expressed genes related to the moth olfactory system, including those encoding the olfactory receptors (ORs) CpomOR1, CpomOR3 and CpomOR6a, which belong to the putative pheromone receptor (PR) lineage, and the co-receptor (CpomOrco). Using heterologous expression, in both human embryonic kidney (HEK293T) cells and in Drosophila olfactory sensory neurons, coupled with calcium imaging and electrophysiological recording, respectively, we characterize the basic physiological and pharmacological properties of these receptors and demonstrate that they form functional ionotropic receptor channels.

Keywords

Codling moth (Cydia pomonella); olfaction; pests

Conference

XXVIIIth annual meeting of the European chemoreception research organization, ECRO 2018, 180905–180909, Würzburg, Germany

SLU Authors

• Cattaneo, Alberto Maria

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    
  • University of Florida

• Gonzalez, Francisco

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Walker, William

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Witzgall, Peter

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology

Permanent link to this page (URI)

https://res.slu.se/id/publ/100343