Recovery of L-Arginine from Model Solutions and Fermentation Broth Using Zeolite-Y Adsorbent

Abstract

Arginine was produced via fermentation of sugars using the engineered microorganism Escherichia coli. Zeolite-Y adsorbents in the form of powder and extrudates were used to recover arginine from both a real fermentation broth and aqueous model solutions. An adsorption isotherm was determined using model solutions and zeolite-Y powder. The saturation loading was determined to be 0.2 g/g using the Sips model. Arginine adsorbed from a real fermentation broth using either zeolite-Y powder or extrudates both showed a maximum loading of 0.15 g/g at pH 11. This adsorbed loading is very close to the corresponding value obtained from the model solution showing that under the experimental conditions the presence of additional components in the broth the adsorption of arginine. Furthermore, a breakthrough curve was determined for extrudates using 1 solution. The selectivity for arginine over ammonia and alanine from the real fermentation broth at pH 11 was 1.9 and 8.3, respectively, for powder, and 1.0, and 4.1, respectively, for extrudates. To the best of our knowledge, this is the first time recovery of arginine from real fermentation broths using any type of adsorbent has been reported.

Keywords

Arginine; FAU; Fermentation broth; Escherichia coli; Adsorption; Selectivity; Breakthrough

Published in

ACS Sustainable Chemistry and Engineering
2019, Volume: 7, number: 9, pages: 8900-8907 Publisher: AMER CHEMICAL SOC

SLU Authors

• Holmlund, Mattias

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Other Chemical Engineering


Publication identifier

DOI: https://doi.org/10.1021/acssuschemeng.9b00918

Permanent link to this page (URI)

https://res.slu.se/id/publ/100362