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Research article - Peer-reviewed, 2019

A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya

Lindahl, Johanna F.; Ragan, Izabela K.; Rowland, R. R.; Wainaina, Martin; Mbotha, Deborah; Wilson, William

Abstract

Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens.

Keywords

Serology; Arbovirus; Vector-borne disease; DIVA test; Seroconversion

Published in

Journal of Virological Methods
2019, Volume: 269, pages: 70-76
Publisher: ELSEVIER SCIENCE BV

    UKÄ Subject classification

    Pathobiology

    Publication Identifiers

    DOI: https://doi.org/10.1016/j.jviromet.2019.04.011

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/100647