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Research article - Peer-reviewed, 2019

Validation of an MPC Polymer Coating to Attenuate Surface-Induced Crosstalk between the Complement and Coagulation Systems in Whole Blood in In Vitro and In Vivo Models

Asif, Sana; Asawa, Kenta; Inoue, Yuuki; Ishihara, Kazuhiko; Lindell, Bjorn; Holmgren, Robin; Nilsson, Bo; Ryden, Anneli; Jensen-Waern, Marianne; Teramura, Yuji; Ekdahl, Kristina N.

Abstract

Artificial surfaces that come into contact with blood induce an immediate activation of the cascade systems of the blood, leading to a thrombotic and/or inflammatory response that can eventually cause damage to the biomaterial or the patient, or to both. Heparin coating has been used to improve hemocompatibility, and another approach is 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymer coatings. Here, the aim is to evaluate the hemocompatibility of MPC polymer coating by studying the interactions with coagulation and complement systems using human blood in vitro model and pig in vivo model. The stability of the coatings is investigated in vitro and MPC polymer-coated catheters are tested in vivo by insertion into the external jugular vein of pigs to monitor the catheters' antithrombotic properties. There is no significant activation of platelets or of the coagulation and complement systems in the MPC polymer-coated one, which was superior in hemocompatibility to non-coated matrix surfaces. The protective effect of the MPC polymer coat does not decline after incubation in human plasma for up to 2 weeks. With MPC polymer-coated catheters, it is possible to easily draw blood from pig for 4 days in contrast to the case for non-coated catheters, in which substantial clotting is seen.

Keywords

blood compatibility; blood model systems; coagulation system; complement system; heparin coat; MPC polymer coat

Published in

Macromolecular Bioscience
2019, Volume: 19, number: 5, article number: 1800485
Publisher: WILEY-V C H VERLAG GMBH

    SLU Authors

    • UKÄ Subject classification

      Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

      Publication Identifiers

      DOI: https://doi.org/10.1002/mabi.201800485

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/100770