Isolation and Detection of Small RNAs from Pollen

Abstract

In the last decade, the accumulation and roles of small RNAs have been slowly uncovered. Recently, the RNA silencing pathways active in the male gametophyte of plants have started to be analyzed in depth. Although several studies have shed light on the small RNA populations present in the pollen, we still lack a clear picture of their regulatory activity. This chapter outlines an extraction method of mature pollen grains and its different downstream applications for the purification and detection of small RNAs.

Keywords

Small RNAs; RNA silencing; Pollen grain; Northern blot; Transposable elements; Sperm cells; Arabidopsis thaliana

Published in

Title: Plant Germline Development
ISBN: 978-1-4939-7285-2, eISBN: 978-1-4939-7286-9Publisher: Humana Press

SLU Authors

• Martinez-Arias, Germán

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany


Publication identifier

DOI: https://doi.org/10.1007/978-1-4939-7286-9_19

Permanent link to this page (URI)

https://res.slu.se/id/publ/100855