Research article - Peer-reviewed, 2006
Porcine circovirus type 2 replicase binds the capsid protein and an intermediate filament-like protein
Timmusk S, Fossum C, Berg MAbstract
Porcine circovirus type 2 (PCV2) is an important porcine pathogen that establishes persistent subclinical infections but may, on activation, contribute to the development of post-weaning multisystemic wasting syndrome (PMWS). This disease is characterized by weight loss, respiratory or digestive disorders and enlarged lymph nodes with lymphocyte depletion. The molecular mechanisms behind the development of the disease are completely unknown. In order to clarify functions of the different viral proteins and, if possible, to connect these new findings to. molecular mechanisms behind the pathogenesis or the viral life cycle, a bacterial two-hybrid screening of a porcine expression library from PK-15A cells was conducted. Using viral proteins corresponding to ORFs 1, 2, 3 and 4 as bait, a number of interactions were identified and two of them were chosen for further characterization. GST pull-down assays confirmed that viral replicase (Rep) interacted with an intermediate filament protein, similar to human syncoilin, and with the transcriptional regulator c-myc. Furthermore, interactions of the viral proteins to each other revealed an interaction between PCV2 Rep and the capsid (Cap) protein and Cap to itselfPublished in
Journal of General Virology2006, volume: 87, pages: 3215-3223
Publisher: SOC GENERAL MICROBIOLOGY
Authors' information
Swedish University of Agricultural Sciences, Department of Molecular Biosciences
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Swedish University of Agricultural Sciences, Department of Forest Mycology and Pathology
Publication Identifiers
DOI: https://doi.org/10.1099/vir.0.81785-0
URI (permanent link to this page)
https://res.slu.se/id/publ/10086