Abstract

There is controversy concerning the presence of xyloglucans in gelatinous (G) layers of Populus spp. tension wood, particularly in mature G-fibers. Transmission electron microscopy (TEM) immunogold localization combined with LM15 antibody (recognizes XXXG-motif of xyloglucans, heptasaccharide) was used to investigate the distribution of xyloglucan epitopes in both transverse and radial sections of P. tremula tension wood. Results provided clear evidence for the presence of xyloglucans in both mature and developing G-layers. Developmental decrease of LM15 epitope localization in G-layers was also detected during G-fiber maturation. High magnification TEM observations showed specific localization of LM15 epitopes on newly synthesized cellulose macrofibrils present in the innermost layer of developing G-layers adjacent to the cell lumen, suggesting linkage between xyloglucans and cellulose macrofibrils. Possible mechanisms were discussed for developmental changes of xyloglucan with respect to the different results reported in the literature.

Keywords

Gelatinous layer; Immunolocalization; LM15 antibody; Populus tremula; Tension wood; Xyloglucan

Published in

Bioresources
2019, Volume: 14, number: 4, pages: 7675-7686

SLU Authors

• Kim, Jongsik

  • Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
    
  • Chonnam National University

• Daniel, Geoffrey

  • Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany
Forest Science
Wood Science


Publication identifier

DOI: https://doi.org/10.15376/biores.14.4.7675-7686

Permanent link to this page (URI)

https://res.slu.se/id/publ/101072