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Research article - Peer-reviewed, 2019

Method for characterizing extracellular proteins from the cell wall proteome of the copper tolerant fungus Phialophora malorum

Daniel, Geoffrey; Volc, Jindrich; Halada, Petr; Karunasekera, Hasanthi; Kim, Jong Sik

Abstract

Phialophora malorum is a well-known copper tolerant fungus causing soft rot decay of copper-treated wood in-service in ground contact situations worldwide. The aim of the present work was to develop a method that allowed studies of the mechanism(s) behind extracellular copper tolerance by examining the profile of proteins expressed by P. malorum in the cell wall/slime (subproteome) environment in mycelia grown in Cu-supplemented media. The study involves development of a novel non-destructive approach using mild extraction, concentration, separation (SDS-PAGE electrophoresis) and characterization (MALDI-TOF MS/MS) of the proteins present. Studies revealed differential protein profiles and expression in Cu-supplemented (0.1% CuSO4) vs control shake cultures after 10 days growth. Twelve proteins including several hydrolytic (i.e. glucosidases, pectinase, polygalacturonase) enzymes were either unique or over-expressed in Cu-cultures compared to controls. The cell wall location of the proteins was consistent with a role in copper tolerance and detoxification by binding Cu-ions extracellularly. Several of the proteins (e.g. hydrolases, pectinase) are also involved in wood cell wall biomineralization. It is proposed that the concentration of metabolites (organic acids) slowly released in close vicinity of hyphal surfaces by weakly bound enzymes over-expressed through Cu-stress may function to reduce levels of free non-chelated Cu (II) ions entering the cell cytoplasm and therefore provide a first level of defense against copper toxicity.

Keywords

Copper tolerance; Differential protein profiles; MALDI-TOF MS/MS; Peptide sequencing; Phialophora malorum; Soft rot decay; Cell wall proteome

Published in

International Biodeterioration and Biodegradation
2019, volume: 144, article number: 104769

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Biomaterials and Technology
Volc, Jindrich
The Czech Academy of Sciences
Halada, Petr
The Czech Academy of Sciences
Karunasekera, Hasanthi
Swedish University of Agricultural Sciences, Department of Forest Biomaterials and Technology
Kim, Jong Sik (Kim, Jong Sik)
Chonnam National University

UKÄ Subject classification

Microbiology
Wood Science

Publication Identifiers

DOI: https://doi.org/10.1016/j.ibiod.2019.104769

URI (permanent link to this page)

https://res.slu.se/id/publ/101433