Research article - Peer-reviewed, 2019
Urine 5-hydroxyindoleacetic acid in Cavalier King Charles spaniels with preclinical myxomatous mitral valve disease
Christiansen, L. B.; Cremer, S. E.; Helander, A.; Madsena, Tine; Reimann, M. J.; Moller, J. E.; Hoglund, K.; Ljungvall, I; Haggstrom, J.; Olsen, L. H.Abstract
Higher concentrations of circulating serotonin have been reported in Cavalier King Charles spaniels (CKCS) compared to other dog breeds. The CKCS is also a breed highly predisposed to myxomatous mitral valve disease (MMVD). The aim of this study was to determine urine concentrations of 5-hydroxyindoleacetic acid (5-HIAA), the major metabolite and excretion product of serotonin, in a population of CKCS with preclinical MMVD, and to evaluate whether urine 5-HIAA concentrations were associated with MMVD severity, dog characteristics, setting for urine sampling, platelet count, and serotonin concentration in serum and platelet-poor plasma (PPP). The study population consisted of 40 privately-owned CKCS (23 females; 17 males) with and without preclinical MMVD as follows: American College of Veterinary Internal Medicine (ACVIM) group A (n =11), ACVIM group B1 (n = 21) and ACVIM group B2 (n = 8).Urine 5-HIAA concentrations were not significantly associated with preclinical MMVD disease, platelet count or circulating concentrations of serotonin (in serum and PPP; P> 0.05). Females had higher 5-HIAA concentrations than males in morning urine collected at home (females, 3.1 [2.9-3.7] mu mol/mmol creatinine [median and quartiles]; males, 1.7 [1.2-2.2] creatinine; P= 0.0002) and urine collected at the clinic (females, 3.5 [3.1-3.9] mu mol/mmol creatinine; males, 1.6 [1.3-2.1] mu mol/mmol creatinine; P < 0.0001). Five-HIAA concentrations in urine collected at home and at the clinic were significantly associated (P= 0.0004; r= 0.73), and higher concentrations were found in urine collected at the clinic (P= 0.013). Urine 5-HIAA concentration was influenced by sex and setting of urine sampling. Urine 5-HIAA concentration was not associated with MMVD severity or circulating concentrations of serotonin in CKCS with preclinical disease. (C) 2019 Elsevier Ltd. All rights reserved.Keywords
5-HIAA; Biomarker; Canine; Mitral valve disease; Serotonin metabolitePublished in
Veterinary Journal2019, volume: 250, pages: 36-43
Publisher: ELSEVIER SCI LTD
Authors' information
Christiansen, L. B.
University of Copenhagen
Cremer, S. E.
University of Copenhagen
Helander, A.
Karolinska Institutet
Madsena, Tine
University of Copenhagen
Reimann, M. J.
University of Copenhagen
Moller, J. E.
Odense University Hospital
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Olsen, L. H.
University of Copenhagen
UKÄ Subject classification
Clinical Science
Publication Identifiers
DOI: https://doi.org/10.1016/j.tvjl.2019.06.005
URI (permanent link to this page)
https://res.slu.se/id/publ/101544