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Research article2019Peer reviewed

The Peptide AmPep1 Derived from Amaranth Recognizes the Replication Hairpin of TYLCV Disturbing Its Replication Process in Host Plants

Mendoza-Figueroa, Jose S.; Badillo-Ramirez, Isidro; Kvarnheden, Anders; Rosas-Ramirez, Daniel G.; Rodriguez-Negrete, Edgar A.; Mendez-Lozano, Jesus; Saniger, Jose M.; Soriano-Garcia, Manuel


Antiviral compounds targeting viral replicative processes have been studied as an alternative for the control of begomoviruses. Previously, we have reported that the peptide AmPep1 has strong affinity binding to the replication origin sequence of tomato yellow leaf curl virus (TYLCV). In this study, we describe the mechanism of action of this peptide as a novel alternative for control of plant-infecting DNA viruses. When AmPep1 was applied exogenously to tomato and Nicotiana benthamiana plants infected with TYLCV, a decrease in the synthesis of the two viral DNA strands (CS and VS) was observed, with a consequent delay in the development of disease progress in treated plants. The chemical mechanism of action of AmPep1 was deduced using Raman spectroscopy and molecular modeling showing the formation of chemical interactions such as H bonds and electrostatic interactions and the formation of pi-pi interactions between both biomolecules contributing to tampering with the viral replication.


peptide; TYLCV; Raman spectroscopy; plant virus; antiviral

Published in

Journal of Agricultural and Food Chemistry
2019, Volume: 67, number: 33, pages: 9241-9253

    Associated SLU-program

    SLU Plant Protection Network
    AMR: Virus

    UKÄ Subject classification

    Structural Biology

    Publication identifier


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