Ethylene Signaling Is Required for Fully Functional Tension Wood in Hybrid Aspen

Abstract

Tension wood (TW) in hybrid aspen trees forms on the upper side of displaced stems to generate a strain that leads to uplifting of the stem. TW is characterized by increased cambial growth, reduced vessel frequency and diameter, and the presence of gelatinous, cellulose-rich (G-)fibers with its microfibrils oriented parallel to the fiber cell axis. Knowledge remains limited about the molecular regulators required for the development of this special xylem tissue with its characteristic morphological, anatomical, and chemical features. In this study, we use transgenic, ethylene-insensitive (ETI) hybrid aspen trees together with time-lapse imaging to show that functional ethylene signaling is required for full uplifting of inclined stems. X-ray diffraction and Raman microspectroscopy of TW in ETI trees indicate that, although G-fibers form, the cellulose microfibril angle in the G-fiber S-layer is decreased, and the chemical composition of S- and G-layers is altered than in wild-type TW. The characteristic asymmetric growth and reduction of vessel density is suppressed during TW formation in ETI trees. A genome-wide transcriptome profiling reveals ethylene-dependent genes in TW, related to cell division, cell wall composition, vessel differentiation, microtubule orientation, and hormone crosstalk. Our results demonstrate that ethylene regulates transcriptional responses related to the amount of G-fiber formation and their properties (chemistry and cellulose microfibril angle) during TW formation. The quantitative and qualitative changes in G-fibers are likely to contribute to uplifting of stems that are displaced from their original position.

Keywords

xylem; wood; ethylene; tension wood; lignin; microfibril angle; Raman microspectroscopy; transcriptomics

Published in

Frontiers in Plant Science
2019, volume: 10, article number: 1101
Publisher: FRONTIERS MEDIA SA

SLU Authors

• Delhomme, Nicolas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Tuominen, Hannele

  • Umeå University

• Sundberg, Björn

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Stora Enso

• Lundberg-Felten, Judith

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Wood Science
Wood Science

Publication identifier

• DOI: https://doi.org/10.3389/fpls.2019.01101

Permanent link to this page (URI)

https://res.slu.se/id/publ/102028