Abstract

From the analytical chemistry point-of-view, an ideal sample preparation method should be simple, rapid, automatic, selective, precise, exhaustive, reproducible and protect the analyte-of-interest from degradation. In this study, a novel sample preparation method, named pressurized matrix solid-phase dispersion (p-MSPD) extraction was developed for simultaneously extracting, separating, purifying, isolating, and analyzing endogenous components in a solid sample matrix. Etlingera elatior, a traditional medicinal plant known as the torch ginger, was applied as a sample matrix to evaluate the p-MSPD process. The entire extraction, separation, isolation, fractionation and detection were performed automatically with a commercial LC-MS system. The novel method was satisfactorily applied for the preparation of real samples without optimization, which had the ability to selectively isolate pure compounds from the solid sample matrix for further NMR analysis. Therefore, the method is recommended for quality control of traditional medicines, research efforts when sample amounts are limited, and laboratories that have ordinary LC-MS instrumentation. (C) 2019 Elsevier B.V. All rights reserved.

Keywords

Etlingera elatior; Pressurized matrix solid-phase dispersion; LC-MS; NMR; Sample preparation

Published in

Journal of Chromatography A
2020, Volume: 1611, article number: 460604

SLU Authors

• Yong, Jean W.H

  • Department of Biosystems and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Analytical Chemistry
Horticulture
Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1016/j.chroma.2019.460604

Permanent link to this page (URI)

https://res.slu.se/id/publ/102039