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Research article2020Peer reviewedOpen access

Diversity and occurrence of chickpea chlorotic dwarf virus on legumes from Iran

Shahmohammadi, N.; Dizadji, A.; Bihamta, R.; Kvarnheden, A.

Abstract

Chickpea chlorotic dwarf virus (CpCDV; genus Mastrevirus, family Geminiviridae) is one of the most important legume-infecting viruses with a wide host range and geographic distribution in Africa and Asia. In Iran, CpCDV is common in chickpea (Cicer arietinum), but there is limited information about diversity and infections in plants of other legume species. In the current study, a total of 1671 leaf samples from different pulse crops with symptoms were collected in nine provinces of Iran, and the CpCDV infection status was tested by PCR and/or rolling circle amplification (RCA), resulting in the detection of CpCDV in samples of chickpea, lentil (Lens culinaris) and faba bean (Vicia faba) from different regions. Sequence analysis of complete genomes of 18 isolates recovered by digestion of RCA products revealed infection with isolates of the strains CpCDV-A and CpCDV-F in chickpea, lentil and faba bean. Phylogenetic analysis showed that the Iranian isolates of CpCDV were closely related to previously sequenced isolates of CpCDV-A and CpCDV-F. To the authors' knowledge, this is the first report of CpCDV-F in Iran. Using agroinoculation with infectious clones for one isolate each of CpCDV-A and CpCDV-F, infectivity was confirmed in both faba bean and chickpea, with plants developing leaf curling and/or yellowing. Both infectious clones also successfully infected Nicotiana benthamiana resulting in mild yellowing and intensive leaf curling for CpCDV-A, and dark-green mosaic, dwarfing and mild leaf curling for CpCDV-F.

Keywords

agroinoculation; CpCDV strain F; genetic diversity; infectious clone; mastrevirus; pulse crops

Published in

Plant Pathology
2020, Volume: 69, number: 1, pages: 139-148 Publisher: WILEY

    Associated SLU-program

    SLU Plant Protection Network

    Sustainable Development Goals

    SDG2 Zero hunger

    UKÄ Subject classification

    Agricultural Science

    Publication identifier

    DOI: https://doi.org/10.1111/ppa.13098

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/102262