Research article2018Peer reviewed
Direct observation of carbohydrate hydroxyl protons in hydrogen bonds with a protein
Nestor, Gustav; Anderson, Taigh; Oscarson, Stefan; Gronenborn, Angela M.
Abstract
Hydroxyl proton resonances of uniformly C-13-labeled Man alpha(1-2)Man alpha(1-2)Man alpha OMe (Man(3)) bound to cyanovirin-N (CV-N) were detected at ambient temperature in aqueous solution by NMR spectroscopy. The directions of the hydroxyl groups were determined on the basis of NOEs, and a previously unknown hydrogen-bonding network between Man(3) and CV-N was discovered. This is the first report on detecting hydroxyl protons of a protein-bound carbohydrate in aqueous solution by NMR. Approaches such as those presented here may open the door for accurately determining intermolecular hydrogen bonds in carbohydrate-protein complexes.
Published in
Journal of the American Chemical Society
2018, Volume: 140, number: 1, pages: 339-345 Publisher: AMER CHEMICAL SOC
UKÄ Subject classification
Structural Biology
Publication identifier
DOI: https://doi.org/10.1021/jacs.7b10595
Permanent link to this page (URI)
https://res.slu.se/id/publ/102271