Abstract

A fluorescent immunochromatographic test strip (FICTS) based on the use of europium nanoparticles (EuNPs) was developed and applied to detect citrinin (CIT) in Monascus fermented food. The sensitivity of the immunoassay to detect CIT was greatly improved by the use of a specific monoclonal antibody to attach EuNPs to form a probe. Under optimum conditions, the visual detection limit was 2.5 ng/mL, and the detection limit of the instrument was 0.05 ng/mL. According to the results, the IC50 was 0.4 ng/mL. Matrix interference from various Monascus fermented foods was investigated in food sample detection. The immunosensor also demonstrated high recoveries (86.8-113.0%) and low relative standard deviations (RSDs) (1.8-15.3%) when testing spiked Monascus fermented food. The detection results of this method showed a good correlation (R-2 > 0.98) with high-performance liquid chromatography (HPLC). The results showed that the FICTS method could be used as a rapid, sensitive method to detect CIT in Monascus fermented food.

Keywords

Citrinin; Fluorescent immunochromatographic assay; Europium nanoparticles; Monascus fermented food

Published in

Toxins
2019, Volume: 11, number: 10, article number: 605
Publisher: MDPI

SLU Authors

• Sun, Chuanxin

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science


Publication identifier

DOI: https://doi.org/10.3390/toxins11100605

Permanent link to this page (URI)

https://res.slu.se/id/publ/102609