Abstract

The pig is commonly used in renal transplantation studies since the porcine kidney resembles the human kidney. To meet the requirements of intense caretaking and examination without stress, a 2-week socialisation and training programme was developed. Conventional cross-breed pigs (n = 36) with high health status were trained for 15 min/day in a four-step training programme before kidney transplantation. The systematic training resulted in calm animals, which allowed for ultrasound examination, blood sampling and urine sampling without restraint. When a 2-methacryloyloxyethyl phosphorylcholine polymer-coated jugular catheter introduced via the auricular vein was used for post-operative blood sampling, clotting was avoided. To assess renal function, urinary output was observed and creatinine and cystatin C were measured; the latter was not found to be useful in recently transplanted pigs. The results presented contribute to the 3Rs (refine, reduce, replace).

Keywords

swine; urinary bladder; indwelling catheter; kidney transplantation

Published in

Laboratory Animals
2020, Volume: 54, number: 5, article number: 0023677219879169
Publisher: SAGE PUBLICATIONS INC

SLU Authors

• Rydén, Anneli

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Manell, Elin

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Hedenqvist, Patricia

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Ley, Charles

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Hansson, Kerstin

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Nyman, Görel

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Jensen Waern, Marianne

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Clinical Science


Publication identifier

DOI: https://doi.org/10.1177/0023677219879169

Permanent link to this page (URI)

https://res.slu.se/id/publ/102617