Desta, Zeratsion Abera
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Desta, Zeratsion Abera; Kolano, Bozena; Shamim, Zeeshan; Armstrong, Susan J.; Rewers, Monika; Sliwinska, Elwira; Kushwaha, Sandeep Kumar; Parkin, Isobel A. P.; Ortiz, Rodomiro; de Koning, Dirk-Jan
Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting annotated genome assemblies and positional cloning of candidate genes. Our principal goal was to construct a genetic map using integrated approaches of genetic, comparative and cytogenetic map analyses. In total, 503 F-2 interspecific hybrid individuals were genotyped using 7,624 single nucleotide polymorphism markers. Comparative analysis demonstrated that similar to 57% of the sequenced loci in L. campestre were congruent with Arabidopsis thaliana (L.) genome and suggested a novel karyotype, which predates the ancestral crucifer karyotype. Aceto-orcein chromosome staining and fluorescence in situ hybridization (FISH) analyses confirmed that L. campestre, L. heterophyllum Benth. and their hybrids had a chromosome number of 2n = 2x = 16. Flow cytometric analysis revealed that both species possess 2C roughly 0.4 picogram DNA. Integrating linkage and comparative maps with cytogenetic map analyses assigned two linkage groups to their particular chromosomes. Future work could incorporate FISH utilizing A. thaliana mapped BAC clones to allow the chromosomes of field cress to be identified reliably.
Lepidium campestre L.; Field cress; genome mapping; oilseed plants
2019, Volume: 9, article number: 17028
Bioinformatics and Systems Biology