Abstract

Enterocytozoon hepatopenaei (EHP) is a pathogen in the pancreatic tissue of prawn, as reported in recent years. Enterosporidiosis caused by EHP in Penaeus genus is spreading widely, which seriously threatens the sustainable development of shrimp aquaculture in the world. Empolying the DNA binding dye of SYTO-16, a real-time quantitative loop-mediated isothermal amplification (LAMP) method has been established herein to detect EHP. The primer sequences used in the LAMP reaction were according to the SSU rRNA gene. The LAMP assay has reached a sensitivity of 10(1) copies/mu L and no cross-reaction with other aquatic pathogens. Compared with normal PCR, the efficiency of the LAMP technique is more sensitive, which has a shorter detection time. The use of fluorescent nucleic acid dye (SYTO-16) reveals a more satisfactory performance relative to calcein. The quantitative LAMP assay can be considered as a valid tool for rapid detection of microsporidian in prawns. Our study provides a scientific basis to follow the effect of the pathogen infection on growth of cultured penaeid shrimp.

Published in

Scientific Reports
2019, Volume: 9, article number: 15860
Publisher: NATURE PUBLISHING GROUP

SLU Authors

• Sun, Chuanxin

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology


Publication identifier

DOI: https://doi.org/10.1038/s41598-019-52459-0

Permanent link to this page (URI)

https://res.slu.se/id/publ/102748