Skip to main content
SLU publication database (SLUpub)

Research article2019Peer reviewedOpen access

Bacillus velezensis 5113 Induced Metabolic and Molecular Reprogramming during Abiotic Stress Tolerance in Wheat

Abd El-Daim, Islam A.; Bejai, Sarosh; Meijer, Johan

Abstract

Abiotic stresses are main limiting factors for agricultural production around the world. Plant growth promoting rhizobacteria (PGPR) have been shown to improve abiotic stress tolerance in several plants. However, the molecular and physiological changes connected with PGPR priming of stress management are poorly understood. The present investigation aimed to explore major metabolic and molecular changes connected with the ability of Bacillus velezensis 5113 to mediate abiotic stress tolerance in wheat. Seedlings treated with Bacillus were exposed to heat, cold/freezing or drought stress. Bacillus improved wheat survival in all stress conditions. SPAD readings showed higher chlorophyll content in 5113-treated stressed seedlings. Metabolite profiling using NMR and ESI-MS provided evidences for metabolic reprograming in 5113-treated seedlings and showed that several common stress metabolites were significantly accumulated in stressed wheat. Two-dimensional gel electrophoresis of wheat leaves resolved more than 300 proteins of which several were differentially expressed between different treatments and that cold stress had a stronger impact on the protein pattern compared to heat and drought. Peptides maps or sequences were used for database searches which identified several homologs. The present study suggests that 5113 treatment provides systemic effects that involve metabolic and regulatory functions supporting both growth and stress management.

Published in

Scientific Reports
2019, Volume: 9, article number: 16282
Publisher: NATURE PUBLISHING GROUP

      SLU Authors

    • Abd El-Daim, Islam Ahmed Moustafa

        • UKÄ Subject classification

        Agricultural Science

        Publication identifier

        DOI: https://doi.org/10.1038/s41598-019-52567-x

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/102803