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Research article - Peer-reviewed, 2020

Disparate effects of antibiotic-induced microbiome change and enhanced fitness in Daphnia magna

Motiei, Asa; Brindefalk, Björn; Ogonowski, Martin; El-Shehawy, Rehab; Pastuszek, Paulina; Ek, Karin; Liewenborg, Birgitta; Udekwu, Klas; Gorokhova, Elena

Abstract

It is a common view that an organism's microbiota has a profound influence on host fitness; however, supporting evidence is lacking in many organisms. We manipulated the gut microbiome of Daphnia magna by chronic exposure to different concentrations of the antibiotic Ciprofloxacin (0.01-1 mg L-1), and evaluated whether this affected the animals fitness and antioxidant capacity. In line with our expectations, antibiotic exposure altered the microbiome in a concentration-dependent manner. However, contrary to these expectations, the reduced diversity of gut bacteria was not associated with any fitness detriment. Moreover, the growth-related parameters correlated negatively with microbial diversity; and, in the daphnids exposed to the lowest Ciprofloxacin concentrations, the antioxidant capacity, growth, and fecundity were even higher than in control animals. These findings suggest that Ciprofloxacin exerts direct stimulatory effects on growth and reproduction in the host, while microbiome- mediated effects are of lesser importance. Thus, although microbiome profiling of Daphnia may be a sensitive tool to identify early effects of antibiotic exposure, disentangling direct and microbiome-mediated effects on the host fitness is not straightforward.

Keywords

gut microbiome; Daphnia magna; antibiotics; Ciprofloxacin; fitness

Published in

PLoS ONE
2020, volume: 15, number: 1, article number: e0214833

Authors' information

Motiei, Asa
Stockholm University
Brindefalk, Björn
Stockholm University
Stockholm University
AquaBiota Water Research
Swedish University of Agricultural Sciences, Department of Aquatic Resources
El-Shehawy, Rehab
Stockholm University
Pastuszek, Paulina
Stockholm University
Ek, Karin
Stockholm University
Liewenborg, Birgitta
Stockholm University
Udekwu, Klas
Stockholm University
Gorokhova, Elena
Stockholm University

UKÄ Subject classification

Biochemistry and Molecular Biology
Microbiology
Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1371/journal.pone.0214833

URI (permanent link to this page)

https://res.slu.se/id/publ/103301