Abstract

Understanding population structure and genetic diversity within and between local Nile tilapia lines cultured in Tanzania is important for sustainable aquaculture production. This study investigated the genetic structure and diversity among seven Nile tilapia populations in Tanzania (Karanga, Igunga, Ruhila, Fisheries Education and Training Agency, Tanzania Fisheries Research Institute, Kunduchi, and Lake Victoria). Double-digest restriction site-associated DNA (ddRAD) libraries were prepared from 140 individual fish (20 per population) and sequenced using an Illumina HiSeq 4000 resulting in the identification of 2,180 informative single nucleotide polymorphisms (SNPs). Pairwise F-st values revealed strong genetic differentiation between the closely related populations; FETA, Lake Victoria, and Igunga and those from TAFIRI and Karanga with values ranging between 0.45 and 0.55. Population structure was further evaluated using Bayesian model-based clustering (STRUCTURE) and discriminant analysis of principal components (DAPC). Admixture was detected among Karanga, Kunduchi, and Ruhila populations. A cross-validation approach (25% of individual fish from each population was considered of unknown origin) was conducted in order to test the efficiency of the SNP markers to correctly assign individual fish to the population of origin. The cross-validation procedure was repeated 10 times resulting in 77% of the tested individual fish being allocated to the correct population. Overall our results provide a new database of informative SNP markers for both conservation management and aquaculture activities of Nile tilapia strains in Tanzania.

Keywords

aquaculture; population structure; genetic diversity; Nile tilapia; double-digest restriction site-associated DNA-sequencing

Published in

Frontiers in Genetics
2019, Volume: 10, article number: 1269
Publisher: FRONTIERS MEDIA SA

SLU Authors

• Kajungiro, Redempta Athanas

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • University of Dar es Salaam

• Palaiokostas, Christos

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Lopes Pinto, Fernando A

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• de Koning, Dirk-Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Sustainable Development Goals

Conserve and sustainably use the oceans, seas and marine resources for sustainable development


UKÄ Subject classification

Fish and Aquacultural Science


Publication identifier

DOI: https://doi.org/10.3389/fgene.2019.01269

Permanent link to this page (URI)

https://res.slu.se/id/publ/103856