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Research article - Peer-reviewed, 2020

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Li, Jiali; Ma, Biao; Fang, Jiehong; Zhi, Antong; Chen, Erjing; Xu, Ying; Yu, Xiaoping; Sun, Chuanxin; Zhang, Mingzhou


Salmonella can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of Salmonella in food. The conserved fragment (fimY) was selected as the target gene. Under an optimal condition (37 degrees C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in a pure culture. Testing with 16 non-Salmonella strains as controls revealed that LFD-RPA was specific to the fimY gene of Salmonella. The established assay could detect Salmonella at concentrations as low as 1.29 x 10(2) CFU/mL in artificially contaminated samples. This detection was at a slightly higher level than that for a pure bacterial culture. Combined with the test strip reader, the LFD-RPA is a feasible method for quantitative detection of Salmonella based on the test line intensity, which was the ratio for the test line and control line of the reflected light. The method could be a potential point-of-care test in limited resource areas and provides a new approach and technical support for the diagnosis of food safety.


recombinase polymerase amplification; lateral flow dipstick; Salmonella; rapid and quantitative detection

Published in

2020, volume: 9, number: 1, article number: 27
Publisher: MDPI

Authors' information

Li, Jiali
China Jiliang Univ
Ma, Biao
China Jiliang Univ
Fang, Jiehong
China Jiliang Univ
Zhi, Antong
China Jiliang Univ
Chen, Erjing
China Jiliang Univ
Xu, Ying
China Jiliang Univ
Yu, Xiaoping
China Jiliang Univ
Swedish University of Agricultural Sciences, Department of Plant Biology
Zhang, Mingzhou
China Jiliang Univ

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