Skip to main content
Research article - Peer-reviewed, 2020

Solvent and Rotating Bed Reactor Extraction with One- and Two-Phase Solvents Applied to Bilberries (Vaccinium myrtillus) for Isolating Valuable Antioxidants

Nystrom, Josefina; Ostman, Ulla-Britt; Mossing, Torgny; Hed, Leif; Geladi, Paul

Abstract

Extraction of antioxidants from bilberries using solvent extraction and the novel rotating bed reactor (RBR) both with one-phase (ethanol and water) and two-phase (ethanol and water +salt) solvents was studied. Solids, ethanol, and temperature settings in an experimental design were monitored for 1 h. The measured responses were (1) polyphenol concentration, (2) visible-near infrared spectra, and (3) HPLC measurement. The (1) responses were used for making response surfaces in time and the spectra (2) could confirm these results. The HPLC results (3) confirmed the results of 1 and 2 but were found unsuitable for online monitoring. The RBR was better than traditional extraction and 16 min sufficed. The response surfaces showed an optimal concentration of ethanol, temperatures above 50 degrees C gave the best results, and high loads of solid were beneficial. Two-phase extraction was less efficient. The methodology could be transferred to larger scale extraction systems to improve yield and save on reagents/energy cost.

Keywords

Folin-Ciocalteu reagent; Total phenolics; Anthocyanidin; Visible and near infrared spectroscopy; HPLC determination; Rotating bed extraction; Two-phase extraction

Published in

Food Analytical Methods
2020, volume: 13, number: 4
Publisher: SPRINGER

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Biomaterials and Technology
Swedish University of Agricultural Sciences, Department of Forest Biomaterials and Technology
Swedish University of Agricultural Sciences, Department of Forest Biomaterials and Technology
Hed, Leif
Centria Univ Appl Sci
Swedish University of Agricultural Sciences, Department of Forest Biomaterials and Technology

Sustainable Development Goals

SDG7 Affordable and clean energy

UKÄ Subject classification

Food Science

Publication Identifiers

DOI: https://doi.org/10.1007/s12161-020-01701-x

URI (permanent link to this page)

https://res.slu.se/id/publ/104884