Lopes Pinto, Fernando A
- Uppsala University
Abstract
Background: Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination.Results: The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are: 1) suitable for a specific organism, and 2) compatible with other oligonucleotides to be used in the experimental procedures.Conclusion: This new web tool allows scientists to easily and rapidly obtain suitable tags for RTPCR experiments, and is available at http://www.egs.uu.se/software/webtag/.
Published in
BMC Biotechnology
2007, volume: 7, article number: 73
SLU Authors
UKÄ Subject classification
Cell and Molecular Biology
Publication identifier
- DOI: https://doi.org/10.1186/1472-6750-7-73
Permanent link to this page (URI)
https://res.slu.se/id/publ/104980