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Research article2020Peer reviewedOpen access

SCHENGEN receptor module drives localized ROS production and lignification in plant roots

Fujita, Satoshi; De Bellis, Damien; Edel, Kai H.; Koester, Philipp; Andersen, Tonni Grube; Schmid-Siegert, Emanuel; Tendon, Valerie Denervaud; Pfister, Alexandre; Marhavy, Peter; Ursache, Robertas; Doblas, Veronica G.; Barberon, Marie; Daraspe, Jean; Creff, Audrey; Ingram, Gwyneth; Kudla, Joerg; Geldner, Niko

Abstract

Production of reactive oxygen species (ROS) by NADPH oxidases (NOXs) impacts many processes in animals and plants, and many plant receptor pathways involve rapid, NOX-dependent increases of ROS. Yet, their general reactivity has made it challenging to pinpoint the precise role and immediate molecular action of ROS. A well-understood ROS action in plants is to provide the co-substrate for lignin peroxidases in the cell wall. Lignin can be deposited with exquisite spatial control, but the underlying mechanisms have remained elusive. Here, we establish a kinase signaling relay that exerts direct, spatial control over ROS production and lignification within the cell wall. We show that polar localization of a single kinase component is crucial for pathway function. Our data indicate that an intersection of more broadly localized components allows for micrometer-scale precision of lignification and that this system is triggered through initiation of ROS production as a critical peroxidase co-substrate.

Keywords

extracellular diffusion barriers; Casparian strips; lignin; localized ROS production; polarized signaling

Published in

EMBO Journal
2020, Volume: 39, number: 9, article number: e103894
Publisher: WILEY