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Research article - Peer-reviewed, 2020

Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection

Aboshady, Hadeer M.; Mandonnet, Nathalie; Felicite, Yoann; Hira, Julien; Fourcot, Aurore; Barbier, Claude; Johansson, Anna M.; Jonas, Elisabeth; Bambou, Jean-Christophe

Abstract

Gastrointestinal nematode (GIN) infections are one of the major constraints for grazing sheep and goat production worldwide. Genetic selection for resistant animals is a promising control strategy. Whole-transcriptome analysis via RNA-sequencing (RNA-seq) provides knowledge of the mechanisms responsible for complex traits such as resistance to GIN infections. In this study, we used RNA-seq to monitor the dynamics of the response of the abomasal mucosa of Creole goat kids infected with Haemonchus contortus by comparing resistant and susceptible genotypes. A total of 8 cannulated kids, 4 susceptible and 4 resistant to GIN, were infected twice with 10 000 L3 H. contortus. During the second infection, abomasal mucosal biopsies were collected at 0, 8, 15 and 35 days post-infection (dpi) from all kids for RNA-seq analysis. The resistant animals showed early activation of biological processes related to the immune response. The top 20 canonical pathways of differentially expressed genes for different comparison showed activation of the immune response through many relevant pathways including the Th1 response. Interestingly, our results showed a simultaneous time series activation of Th2 related genes in resistant compared to susceptible kids.

Keywords

Gastrointestinal nematode (GIN) infections; sheep; goats; genetic selection; resistance

Published in

Veterinary Research
2020, volume: 51, number: 1, article number: 44
Publisher: BMC

Authors' information

Aboshady, Hadeer M. (Aboshady, Hadeer M.)
AgroParisTech
Aboshady, Hadeer M. (Aboshady, Hadeer M.)
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Aboshady, Hadeer Moursy (Aboshady, Hadeer Moursy)
INRAE
Aboshady, Hadeer M. (Aboshady, Hadeer M.)
Cairo University
Mandonnet, Nathalie
INRAE
Felicite, Yoann
INRAE
Hira, Julien
INRAE
Fourcot, Aurore
INRAE
Barbier, Claude
INRAE
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Jonas, Elisabeth
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Bambou, Jean-Christophe
INRAE

UKÄ Subject classification

Clinical Science

Publication Identifiers

DOI: https://doi.org/10.1186/s13567-020-00768-y

URI (permanent link to this page)

https://res.slu.se/id/publ/105155