Research article - Peer-reviewed, 2020
The Mitogenome of Norway Spruce and a Reappraisal of Mitochondrial Recombination in PlantsSullivan, Alexis R.; Eldfjell, Yrin; Schiffthaler, Bastian; Delhomme, Nicolas; Asp, Torben; Hebelstrup, Kim H.; Keech, Olivier; Oberg, Lisa; Moller, Ian Max; Arvestad, Lars; Street, Nathaniel R.; Wang, Xiao-Ru
AbstractPlant mitogenomes can be difficult to assemble because they are structurally dynamic and prone to intergenomic DNA transfers, leading to the unusual situation where an organelle genome is far outnumbered by its nuclear counterparts. As a result, comparative mitogenome studies are in their infancy and some key aspects of genome evolution are still known mainly from pregenomic, qualitative methods. To help address these limitations, we combined machine learning and in silico enrichment of mitochondrial-like long reads to assemble the bacterial-sized mitogenome of Norway spruce (Pinaceae: Picea abies). We conducted comparative analyses of repeat abundance, intergenomic transfers, substitution and rearrangement rates, and estimated repeat-by-repeat homologous recombination rates. Prompted by our discovery of highly recombinogenic small repeats in P. abies, we assessed the genomic support for the prevailing hypothesis that intramolecular recombination is predominantly driven by repeat length, with larger repeats facilitating DNA exchange more readily. Overall, we found mixed support for this view: Recombination dynamics were heterogeneous across vascular plants and highly active small repeats (ca. 200 bp) were present in about one-third of studied mitogenomes. As in previous studies, we did not observe any robust relationships among commonly studied genome attributes, but we identify variation in recombination rates as a underinvestigated source of plant mitogenome diversity.
Keywordsmitogenome; repeats; recombination; rearrangement rates; structural variation
Published inGenome Biology and Evolution
2020, volume: 12, number: 1, pages: 3586-3598
Publisher: OXFORD UNIV PRESS
Sullivan, Alexis R.
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Hebelstrup, Kim H.
Oldtjikko Photo Art and Sci
Moller, Ian Max
Street, Nathaniel R.
UKÄ Subject classification
URI (permanent link to this page)