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Research article - Peer-reviewed, 2020

Assigned NMR backbone resonances of the ligand-binding region domain of the pneumococcal serine-rich repeat protein (PsrP-BR) reveal a rigid monomer in solution

Schulte, Tim; Sala, Benedetta Maria; Nilvebrant, Johan; Nygren, Per-Ake; Achour, Adnane; Shernyukov, Andrey; Agback, Tatiana; Agback, Peter


The pneumococcal serine rich repeat protein (PsrP) is displayed on the surface of Streptococcus pneumoniae with a suggested role in colonization in the human upper respiratory tract. Full-length PsrP is a 4000 residue-long multi-domain protein comprising a positively charged functional binding region (BR) domain for interaction with keratin and extracellular DNA during pneumococcal adhesion and biofilm formation, respectively. The previously determined crystal structure of the BR domain revealed a flat compressed barrel comprising two sides with an extended beta-sheet on one side, and another beta-sheet that is distorted by loops and beta-turns on the other side. Crystallographic B-factors indicated a relatively high mobility of loop regions that were hypothesized to be important for binding. Furthermore, the crystal structure revealed an inter-molecular beta-sheet formed between edge strands of two symmetry-related molecules, which could promote bacterial aggregation during biofilm formation. Here we report the near complete N-15/C-13/H-1 backbone resonance assignment of the BR domain of PsrP, revealing a secondary structure profile that is almost identical to the X-ray structure. Dynamic N-15-T-1, T-2 and NOE data suggest a monomeric and rigid structure of BR with disordered residues only at the N- and C-termini. The presented peak assignment will allow us to identify BR residues that are crucial for ligand binding.


NMR assignments; Pneumococcal serine rich repeat protein; Secondary structure; X-ray comparison; Backbone dynamics

Published in

Biomolecular NMR Assignments
2020, volume: 14, number: 2, pages: 195-200
Publisher: SPRINGER

Authors' information

Schulte, Tim
Karolinska Univ Hosp
Sala, Benedetta Maria
Karolinska Univ Hosp
Nilvebrant, Johan
AlbaNova Univ Ctr
Nygren, Per-Ake
AlbaNova Univ Ctr
Achour, Adnane
Karolinska Univ Hosp
Sherniukov, Andrei
Vorozhtsov Novosibirsk Institute of Organic Chemistry
Sherniukov, Andrei
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Swedish University of Agricultural Sciences, Department of Molecular Sciences

UKÄ Subject classification

Cell and Molecular Biology

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