Glutamate over-accumulation may serve as an endogenous indicator of tricarboxylic acid (TCA) cycle suppression under NH4+ nutrition in wheat (Triticum aestivum L.) seedlings

Abstract

Soil excess ammonium (NH4+) has serious negative effects on crop growth and yield formation. Most studies of NH4+ stress have addressed only the effects of free NH4+, failing to recognize changes in nitrogen (N) assimilation products. Hydroponic experiments were conducted using 5 mM NH4+ or nitrate (NO3-) forms of N in two wheat cultivars that differed in NH4+ tolerance (NH4+-sensitive AK58 and NH4+-tolerant XM25). To evaluate the effects of NH4+ assimilation products on plant growth, 1 mu M L-methionine sulfoximine (MSO, an inhibitor of glutamine synthetase) and 1 mM glutamate (a primary N assimilation product) were added to the N source solutions. NH4+ treatments significantly reduced plant biomass in both cultivars, but to a greater extent in AK58. These inhibition effects were alleviated by MSO and strengthened by the application of glutamate. The free NH4+ concentration was increased under NH4+ conditions and was enhanced by the application of MSO. Amino acids levels were significantly greater in both cultivars; however, XM25 showed a lower glutamate concentration and a lower ratio of (glutamate + glutamine) to (aspartic acid + asparagine) than AK58. Under NH4+ conditions, levels of tricarboxylic acid (TCA) cycle intermediates and adenosine triphosphate were decreased due to inhibited pyruvate kinase activity; this inhibition was enhanced by the application of glutamate, but relieved by MSO. These results show that, aside from the large amounts of free NH4+ in wheat tissues, as a primary N assimilation product, the over-accumulated glutamate is also involved in interrupting TCA cycle metabolism under NH4+ stress, leading to reduced plant growth.

Keywords

Ammonium stress; Glutamate; Tricarboxylic acid cycle; Triticum aestivum

Published in

Environmental and Experimental Botany
2020, Volume: 177, article number: 104130

SLU Authors

• Yong, Jean W.H

  • Department of Biosystems and Technology, Swedish University of Agricultural Sciences
    
  • University of Western Australia

UKÄ Subject classification

Soil Science
Botany
Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1016/j.envexpbot.2020.104130

Permanent link to this page (URI)

https://res.slu.se/id/publ/105634