Research article - Peer-reviewed, 2015
Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus
Braesel, Jana; Goetze, Sebastian; Shah, Firoz; Heine, Daniel; Tauber, James; Hertweck, Christian; Tunlid, Anders; Stallforth, Pierre; Hoffmeister, DirkAbstract
The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.Published in
Chemistry and Biology2015, volume: 22, number: 10, pages: 1325-1334
Authors' information
Braesel, Jana
Univ Jena
Goetze, Sebastian
Hans Knoell Inst
Lund University
Heine, Daniel
Hans Knoell Inst
Tauber, James
Univ Jena
Hertweck, Christian
Hans Knoell Inst
Tunlid, Anders
Lund Univ
Stallforth, Pierre
Hans Knoell Inst
Hoffmeister, Dirk
Univ Jena
UKÄ Subject classification
Microbiology
Biochemistry and Molecular Biology
Publication Identifiers
DOI: https://doi.org/10.1016/j.chembiol.2015.08.016
URI (permanent link to this page)
https://res.slu.se/id/publ/105907