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Research article2006Peer reviewedOpen access

The Brichos domain-containing C-terminal part of pro-surfactant protein C binds to an unfolded poly-Val transmembrane segment

Johansson H, Nordling K, Weaver TE, Johansson J

Abstract

Native lung surfactant protein C (SP-C) is a 4.2-kDa acylpeptide that associates with alveolar surfactant phospholipids via a transmembrane alpha-helix. This helix contains mainly Val, although poly-Val is inefficient in helix formation, and helical SP-C can spontaneously convert to beta-sheet aggregates and amyloid-like fibrils. SP-C is cleaved out from a 21-kDa integral membrane protein, proSP-C, in the alveolar type II cell. Recently several mutations localized in the endoplasmic reticulum-lumenal (C-terminal) part of proSP-C (CTproSP-C) have been associated with intracellular accumulation of toxic forms of proSP-C, low levels of mature SP-C, and development of interstitial lung disease. CTproSP-C contains a similar to 100-residue Brichos domain of unknown function that is also found in other membrane proteins associated with amyloid formation, dementia, and cancer. Here we find that recombinant CTproSP-C binds lipid-associated SP-C, which is in beta-strand conformation, and that this interaction results in an increased helical content. In contrast, CTproSP-C does not bind alpha-helical SP-C. Recombinant CTproSP-C(L188Q), a mutation associated with interstitial lung disease, shows secondary and quaternary structures similar to those of wild type CTproSP-C but is unable to bind lipid-associated beta-strand SP-C. Transfection of CTproSP-C into HEK293 cells that express proSP-C(L188Q) increases the amount of proSP-C protein, whereas no effect is seen on cells expressing wild type proSP-C. These findings suggest that CTproSP-C binds nonhelical SP-C and thereby prevents beta-sheet aggregation and that mutations in CTproSP-C can interfere with this function

Published in

Journal of Biological Chemistry
2006, Volume: 281, number: 30, pages: 21032-21039
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

      SLU Authors

    • Willander, Hanna

      • Department of Molecular Biosciences, Swedish University of Agricultural Sciences

      • Nordling, Kerstin

        • Department of Molecular Biosciences, Swedish University of Agricultural Sciences

        • Johansson, Jan

          • Department of Molecular Biosciences, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Veterinary Science
        Animal and Dairy Science

        Publication identifier

        DOI: https://doi.org/10.1074/jbc.M603001200

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/10598