Johansson, Jan
- Department of Molecular Biosciences, Swedish University of Agricultural Sciences
Abstract
Using surface plasmon resonance (SPR) and electrospray mass spectrometry (ESI-MS), proinsulin C-peptide was found to influence insulin-insulin interactions. In SPR with chip-bound insulin, C-peptide mixed with analyte insulin increased the binding, while alone C-peptide did not. A control peptide with the same residues in random sequence had little effect. In ESI-MS, C-peptide lowered the presence of insulin hexamer. The data suggest that C-peptide promotes insulin disaggregation. Insulin/insulin oligomer mu M dissociation constants were determined. Compatible with these findings, type 1 diabetic patients receiving insulin and C-peptide developed 66% more stimulation of glucose metabolism than when given insulin alone. A role of C-peptide in promoting insulin disaggregation may be important physiologically during exocytosis of pancreatic beta-cell secretory granulae and pharmacologically at insulin injection sites. It is compatible with the normal co-release of C-peptide and insulin and may contribute to the beneficial effect of C-peptide and insulin replacement in type 1 diabetics.
Keywords
surface plasmon resonance; electrospray ionization mass spectrometry; insulin effect; diabetes type 1; proinsulin C-peptide; insulin disaggregation; insulin hexamer decrease
Published in
Cellular and Molecular Life Sciences
2006, volume: 63, number: 15, pages: 1805-1811
Publisher: BIRKHAUSER VERLAG AG
SLU Authors
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1007/s00018-006-6204-6
Permanent link to this page (URI)
https://res.slu.se/id/publ/10600