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Research article - Peer-reviewed, 2019

Detection of peste des petits ruminants and concurrent secondary diseases in sheep and goats in Ngorongoro district, Tanzania

Chota, Andrew; Chubwa, Choby C.; Nyasebwa, Obed; Lyimo, Beatus; Torsson, Emeli; Karimuribo, Esron; Kasanga, Christopher J.; Johansson Wensman, Jonas; Misinzo, Gerald; Shirima, Gabriel; Kusiluka, Lughano

Abstract

Small ruminants play an important role in the livelihoods of resource-constrained communities. This study was initiated because of a massive outbreak of a respiratory disease in sheep and goats in Loliondo area in Ngorongoro district of Arusha region in Tanzania in 2016. During flock examination, a total of 240 serum samples and 61 nasal swabs were collected. Antibodies to small ruminant morbillivirus, causative agent of peste des petits ruminants (PPR), were detected from sera using a competitive enzymelinked immunosorbent assay. A multiplex reverse transcription real-time polymerase chain reaction assay was used to detect four pathogens: small ruminant morbillivirus, Mycoplasma capricolum subspecies capripneumoniae, Pasteurella multocida, and Capripoxvirus from the nasal swabs. Overall seroprevalence of PPR was 74.6%, with all four pathogens detected from nasal swabs. Co-infections of small ruminant morbillivirus and Mycoplasma capricolum subspecies capripneumoniae, small ruminant morbillivirus and Capripoxvirus, small ruminant morbillivirus and Pasteurella multocida, and Mycoplasma capricolum subspecies capripneumoniae and Capripoxvirus were also detected. Presence of PPR and the other diseases in this study provided insight into the severity of the outbreak in sheep and goats in Ngorongoro district. Thus, laboratory confirmation is critical for prompt and appropriate interventions to be made for control of diseases in sheep and goats with similar clinical signs. The findings also call for research into development of combined vaccines targeting common diseases of small ruminants in Tanzania.

Keywords

PPR; CCPP; Goats; Sheep

Published in

Comparative Clinical Pathology
2019, volume: 28, pages: 755-759

Authors' information

Chota, Andrew
Nelson Mandela African Institution of Science and Technology
Chubwa, Choby C.
No organisation
Nyasebwa, Obed
Veterinary Investigation Centre
Lyimo, Beatus
Nelson Mandela African Institution of Science and Technology
Torsson, Emeli
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Karimuribo, Esron
Sokoine University of Agriculture
Kasanga, Christopher J.
Sokoine University of Agriculture
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Misinzo, Gerald
Sokoine University of Agriculture
Shirima, Gabriel
Nelson Mandela African Institution of Science and Technology
Kusiluka, Lughano
Mzumbe University

UKÄ Subject classification

Pathobiology
Clinical Science

Publication Identifiers

DOI: https://doi.org/10.1007/s00580-018-2848-5

URI (permanent link to this page)

https://res.slu.se/id/publ/106449