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Research article - Peer-reviewed, 2020

The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis

Smith, Stephanie; Zhu, Shanshuo; Joos, Lisa; Roberts, Ianto; Nikonorova, Natalia; Vu, Lam Dai; Stes, Elisabeth; Cho, Hyunwoo; Larrieu, Antoine; Xuan, Wei; Goodall, Benjamin; van de Cotte, Brigitte; Waite, Jessic Marie; Rigal, Adeline; Harborough, Sigurd Ramans; Persiau, Geert; Vanneste, Steffen; Kirschner, Gwendolyn K.; Vandermarliere, Elien; Martens, Lennart;
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Abstract

Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabi-lizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.

Published in

Molecular and Cellular Proteomics
2020, volume: 19, number: 8, pages: 1248-1262

Authors' information

Smith, Stephanie
University of Nottingham
Zhu, Shanshuo
Ghent University
Joos, Lisa
Ghent University
Roberts, Ianto
Ghent University
Nikonorova, Natalia
Ghent University
Vu, Lam Dai
Ghent University
Stes, Elisabeth
Ghent University
Cho, Hyunwoo
Ruprecht Karls University Heidelberg
Larrieu, Antoine
University of Leeds
Xuan, Wei
Ghent University
Goodall, Benjamin
University of Nottingham
van de Cotte, Brigitte
Ghent University
Waite, Jessic Marie
University of Washington
Rigal, Adeline
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Harborough, Sigurd Ramans
University of Leeds
Persiau, Geert
Ghent University
Vanneste, Steffen
Ghent University
Kirschner, Gwendolyn K.
Heinrich Heine University Dusseldorf
Vandermarliere, Elien
Ghent University
Martens, Lennart
Ghent University
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UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.1074/mcp.RA119.001826

URI (permanent link to this page)

https://res.slu.se/id/publ/106503