Abstract

A monoclonal antibody (Mab), Mab 4-24-11, to human class II major histocompatibility antigens has been tested in commonly used immunoassays for detection of porcine class II major histocompatibility antigens (SLA-D).In a radioimmunoprecipitation assay, Mab 4-24-11 reacted with proteins from mitogen-stimulated porcine mononuclear cells (MNC). The molecular weights of the precipitated proteins were approximately 34 and 28 Kd. In indirect immunofluorescence, approximately 25% of porcine MNC in suspension reacted with Mab 4-24-11. This percentage diminished to 14% when Ig bearing MNC were removed, while it increased to 36% when adherent MNC were enriched. Thus, it was concluded that Mab 4-24-11 cross-reacts with SLA-D.In a immunohistochemical study, Mab 4-24-11 reacted with cells in acetone fixed cryostat sections from the gastric mucosa and endometrium.These properties of Mab 4-24-11 make it useful as a tool for further studies on the porcine immune system.

Published in

Journal of veterinary medicine. Series B
1991, Volume: 38, number: 5, pages: 321-328
Publisher: BLACKWELL WISSENSCHAFTS-VERLAG GMBH

SLU Authors

• Magnusson, Ulf

  • Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences
    

• Fossum, Caroline

  • Department of Veterinary Microbiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Clinical Science


Publication identifier

DOI: https://doi.org/10.1111/j.1439-0450.1991.tb00878.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/106576