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Research article - Peer-reviewed, 2007

Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121

Melin P, Hakansson S, Schnurer J

Abstract

The biocontrol yeast Pichia anomala J121 can effectively reduce mould growth on moist cereal grains during airtight storage. Practical use of microorganisms requires formulated products that meet a number of criteria. In this study we compared different formulations of P. anomala. The best way to formulate P. anomala was freeze-drying. The initial viability was as high as 80%, with trehalose previously added to the yeast. Freeze-dried products could be stored at temperatures as high as 30 degrees C for a year, with only a minor decrease in viability. Vacuum-drying also resulted in products with high storage potential, but the products were not as easily rehydrated as freeze-dried samples. Upon desiccating the cells using fluidised-bed drying or as liquid formulations, a storage temperature of 10 degrees C was required to maintain viability. Dependent on the type of formulation, harvesting of cells at different nutritional stresses affected the initial viabilities, e.g. the initial viability for fluidised-bed-dried cells was higher when the culture was fed with excess glucose, but for freeze-drying it was superior when cells were harvested after depletion of carbon. Using micro-silos we found that the biocontrol activity remained intact after drying, storage and rehydration for all formulations

Published in

Applied Microbiology and Biotechnology
2007, Volume: 73, number: 5, pages: 1008-1016

      SLU Authors

      • UKÄ Subject classification

        Agricultural Science
        Food Science
        Environmental Sciences related to Agriculture and Land-use

        Publication identifier

        DOI: https://doi.org/10.1007/s00253-006-0552-x

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/10684