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Research article - Peer-reviewed, 2020

Seed Germination after 30 Years Storage in Permafrost

Solberg, Svein Oivind; Brodal, Guro; von Bothmer, Roland; Meen, Eivind; Yndgaard, Flemming; Andreasen, Christian; Asdal, Asmund


More than 30 years ago, the Nordic Gene Bank established a long-term experiment on seeds stored under permafrost conditions in an abandoned mine corridor in Svalbard, as a tool to monitor storage life under these conditions. The study included seeds from 16 Nordic agricultural and horticultural crops, each represented by two or three cultivars (altogether 38 accessions). All seeds were ultra-dried to 3-5% moisture before being sealed in glass tubes. Germination tests were performed in accordance with the International Seed Testing Association (ISTA) protocols. At the initiation of the experiment, the samples showed good germination with the median value at 92%. The overall picture remained stable over the first twenty to twenty-five years. However, the variation became larger over time and at 30 years, the median value had dropped to 80%. At the lower end, with a high drop in germination, we found rye, wheat, and English ryegrass. At the upper end, we found Kentucky bluegrass and cucumber. The lowest germination was found in samples with the highest initial seed moisture levels. Pre-storage conditions are likely to be of major importance for longevity.


ex situ conservation; germination; longevity; plant genetic resources; seed storage

Published in

2020, volume: 9, number: 5, article number: 579
Publisher: MDPI

Authors' information

Solberg, Svein Øivind
Inland Norway University of Applied Sciences
Brodal, Guro
Norwegian Inst Bioecon Res NIBIO
Swedish University of Agricultural Sciences, Department of Plant Breeding
Meen, Eivind
Kimen Seed Laboratory
Yndgaard, Flemming
Nordic Genetic Resource Center (NordGen)
Andreasen, Christian
University of Copenhagen
Asdal, Åsmund
Nordic Genetic Resource Center (NordGen)

UKÄ Subject classification

Genetics and Breeding

Publication Identifiers


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