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Research article - Peer-reviewed, 2020

CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen

Bunder, Anne; Sundman, Ola; Mahboubi, Amir; Persson, Staffan; Mansfield, Shawn D.; Ruggeberg, Markus; Niittyla, Totte;

Abstract

Cellulose microfibrils synthesized by CELLULOSE SYNTHASE COMPLEXES (CSCs) are the main load-bearing polymers in wood. CELLULOSE SYNTHASE INTERACTING1 (CSI1) connects CSCs with cortical microtubules, which align with cellulose microfibrils. Mechanical properties of wood are dependent on cellulose microfibril alignment and structure in the cell walls, but the molecular mechanism(s) defining these features is unknown. Herein, we investigated the role of CSI1 in hybrid aspen (Populus tremula x Populus tremuloides) by characterizing transgenic lines with significantly reducedCSI1transcript abundance. Reduction in leaves (50-80%) caused leaf twisting and misshaped pavement cells, while reduction (70-90%) in developing xylem led to impaired mechanical wood properties evident as a decrease in the elastic modulus and rupture. X-ray diffraction measurements indicate that microfibril angle was not impacted by the alteredCSI1abundance in developing wood fibres. Instead, the augmented wood phenotype of the transgenic trees was associated with a reduced cellulose degree of polymerization. These findings establish a function for CSI1 in wood mechanics and in defining leaf cell shape. Furthermore, the results imply that the microfibril angle in wood is defined by CSI1 independent mechanism(s).

Keywords

aspen; Populus; cell wall; wood mechanics; cellulose; transgenic trees; cellulose interacting 1; CSI1; pavement cell

Published in

Plant Journal

2020, volume: 103, number: 5, pages: 1858-1868
Publisher: WILEY

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Sundman, Ola
Umea Univ
Mahboubi, Amir
Umea Univ
Persson, Staffan
Univ Melbourne
Mansfield, Shawn D.
Univ British Columbia
Ruggeberg, Markus
Empa
Ruggeberg, Markus
Swiss Fed Inst Technol
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Wood Science

Publication Identifiers

DOI: https://doi.org/10.1111/tpj.14873

URI (permanent link to this page)

https://res.slu.se/id/publ/106990